URMC / Labs / Wedekind Lab / Lab Protocols Lab Protocols RNA In vitro transcription Analytical urea PAGE C18 RNA purification Lyophilization Sep Pak desalting Protein Electrophoresis stocks Protein PAGE recipes T7 prep (non-6His) Molecular Biology Cell growth/transformation Crystallization Joe’s Crystallization Stocks Joe’s 24-well Plate Tracker Torelli’s Home-Data-Set Form Jolanta’s DLS presentation Methods and Reviews RNA Prep, Purif, Crystallization Metal Ion Coordination in RNA Torelli synchrotron-packing list SHELX RNA SAD Phasing RNA Preparation & Derivatives CNS Multi-conformational RNA Ivan's Protocols 8HMBPMS2 Purification Protocol 6HTEV Protease Purification 6H-TEV-U1A Purification Co-crystallization of RNA Molecules with U1A CSB Media Recipe Detailed Western Blotting Protocol Expression in Ecoli using Autoinduction Frameshifting Protocol RRL Gluthathione Resin Regeneration GST-Prescission Protease Purification (GML) In vitro Transcription Recipe Native RNA Purification Protocol Ni-NTA Resin Regeneration Preparing Competent Cells Protocol for Harvesting Pfu (Barrick Lab) PyMOL Structure Preparation Notes Restriction Enzyme End Requirements (NEB) Superscript III RT Purification T7 RNAP Purification TEV Protease Cleavage Protocol VP-ITC Protocol Andrew's Protocols PAGE gel for RNA Deprotection of synthetic RNA from Dharmacon Analytical Gel Post Urea-PAGE DEAE Purification of RNA SEC desalting purification of RNA