URMC / Labs / Kielkopf Lab / Publications Recent Publications Maji DJenkins JLBoutz PLKielkopf CL Recurrent Neurodevelopmentally Associated Variants of the Pre-mRNA Splicing Factor U2AF2 Alter RNA Binding Affinities and Interactions.; Biochemistry. 2024 Oct 10. Galardi JWBela VNJeffery NHe XGlasser ELoerch SJenkins JLPulvino MJBoutz PLKielkopf CL A UHM - ULM interface with unusual structural features contributes to U2AF2 and SF3B1 association for pre-mRNA splicing.; The Journal of biological chemistry. 2022 Jun 30. Glasser EMaji DBiancon GPuthenpeedikakkal AMKCavender CETebaldi TJenkins JLMathews DHHalene SKielkopf CL Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal.; Nucleic acids research. 2022 May 07. Chatrikhi RFeeney CFPulvino MJAlachouzos GMacRae AJFalls ZRai SBrennessel WWJenkins JLWalter MJGraubert TASamudrala RJurica MSFrontier AJKielkopf CL A synthetic small molecule stalls pre-mRNA splicing by promoting an early-stage U2AF2-RNA complex.; Cell chemical biology. 2021 Feb 26. Maji DGlasser EHenderson SGalardi JPulvino MJJenkins JLKielkopf CL Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing.; The Journal of biological chemistry; Vol 295(50). 2020 Dec 11. Maji DGlasser EHenderson SGalardi JPulvino MJJenkins JLKielkopf CL Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing.; The Journal of biological chemistry. 2020 Oct 05. Warnasooriya CFeeney CFLaird KMErmolenko DNKielkopf CL A splice site-sensing conformational switch in U2AF2 is modulated by U2AF1 and its recurrent myelodysplasia-associated mutation.; Nucleic acids research; Vol 48(10). 2020 Jun 04. Maji DGrossfield AMKielkopf CL Structures of SF3b1 reveal a dynamic Achilles heel of spliceosome assembly: Implications for cancer-associated abnormalities and drug discovery.; Biochimica et biophysica acta. Gene regulatory mechanisms. 2019 Nov 07. Talkish JIgel HHunter OHorner SWJeffery NNLeach JRJenkins JLKielkopf CLAres M Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1 through a UHM-ULM interaction.; RNA (New York, N.Y.). 2019 May 20. Kennedy SDBauer WJWang WKielkopf CL Dynamic stacking of an expected branch point adenosine in duplexes containing pseudouridine-modified or unmodified U2 snRNA sites.; Biochemical and biophysical research communications. 2019 Feb 20. Loerch SLeach JRHorner SWMaji DJenkins JLPulvino MKielkopf CL The pre-mRNA splicing and transcription factor Tat-SF1 is a functional partner of the spliceosome SF3b1 subunit via a U2AF homology motif interface.; The Journal of biological chemistry. 2018 Dec 19. Kielkopf CL Insights from structures of cancer-relevant pre-mRNA splicing factors.; Current opinion in genetics & development; Vol 48. 2017 Nov 09. Glasser EAgrawal AAJenkins JLKielkopf CL Cancer-Associated Mutations Mapped on High-Resolution Structures of the U2AF2 RNA Recognition Motifs.; Biochemistry. 2017 Sep 01. Jenkins JLKielkopf CL Splicing Factor Mutations in Myelodysplasias: Insights from Spliceosome Structures.; Trends in genetics : TIG. 2017 Mar 31. Chatrikhi RWang WGupta ALoerch SMaucuer AKielkopf CL SF1 Phosphorylation Enhances Specific Binding to U2AF(65) and Reduces Binding to 3'-Splice-Site RNA.; Biophysical journal; Vol 111(12). 2016 Dec 20. Loerch SKielkopf CL Unmasking the U2AF homology motif family: a bona fide protein-protein interaction motif in disguise.; RNA (New York, N.Y.); Vol 22(12). 2016 Dec. Fei DLMotowski HChatrikhi RPrasad SYu JGao SKielkopf CLBradley RKVarmus H Wild-Type U2AF1 Antagonizes the Splicing Program Characteristic of U2AF1-Mutant Tumors and Is Required for Cell Survival.; PLoS genetics; Vol 12(10). 2016 Oct. Agrawal AASalsi EChatrikhi RHenderson SJenkins JLGreen MRErmolenko DNKielkopf CL An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.; Nature communications; Vol 7. 2016. Loerch SKielkopf CL Dividing and Conquering the Family of RNA Recognition Motifs: A Representative Case Based on hnRNP L.; Journal of molecular biology; Vol 427(19). 2015 Sep 25. Okeyo-Owuor TWhite BSChatrikhi RMohan DRKim SGriffith MDing LKetkar-Kulkarni SHundal JLaird KMKielkopf CLLey TJWalter MJGraubert TA U2AF1 mutations alter sequence specificity of pre-mRNA binding and splicing.; Leukemia; Vol 29(4). 2015 Apr. Agrawal AAMcLaughlin KJJenkins JLKielkopf CL Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA.; Proceedings of the National Academy of Sciences of the United States of America; Vol 111(49). 2014 Dec 9. Loerch SMaucuer AManceau VGreen MRKielkopf CL Cancer-relevant splicing factor CAPERα engages the essential splicing factor SF3b155 in a specific ternary complex.; The Journal of biological chemistry; Vol 289(25). 2014 Jun 20. Jenkins JLAgrawal AAGupta AGreen MRKielkopf CL U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs.; Nucleic acids research; Vol 41(6). 2013 Apr 1. Gleghorn MLGong CKielkopf CLMaquat LE Staufen1 dimerizes through a conserved motif and a degenerate dsRNA-binding domain to promote mRNA decay.; Nature structural & molecular biology; Vol 20(4). 2013 Apr. Wang WMaucuer AGupta AManceau VThickman KRBauer WJKennedy SDWedekind JEGreen MRKielkopf CL Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex.; Structure (London, England : 1993); Vol 21(2). 2013 Feb 5. Jenkins JLLaird KMKielkopf CL A Broad range of conformations contribute to the solution ensemble of the essential splicing factor U2AF(65).; Biochemistry; Vol 51(26). 2012 Jul 3. Bauer WJHeath JJenkins JLKielkopf CL Three RNA recognition motifs participate in RNA recognition and structural organization by the pro-apoptotic factor TIA-1.; Journal of molecular biology; Vol 415(4). 2012 Jan 27. Gupta AKielkopf CL Purification, crystallization and preliminary X-ray crystallographic analysis of a central domain of human splicing factor 1.; Acta crystallographica. Section F, Structural biology and crystallization communications; Vol 67(Pt 4). 2011 Apr 01. McLaughlin KJJenkins JLKielkopf CL Large favorable enthalpy changes drive specific RNA recognition by RNA recognition motif proteins.; Biochemistry; Vol 50(9). 2011 Mar 8. Gupta AJenkins JLKielkopf CL RNA induces conformational changes in the SF1/U2AF65 splicing factor complex.; Journal of molecular biology; Vol 405(5). 2011 Feb 04. McLaughlin KJStrain-Damerell CMXie KBrekasis DSoares ASPaget MSKielkopf CL Structural basis for NADH/NAD+ redox sensing by a Rex family repressor.; Molecular cell; Vol 38(4). 2010 May 28. Jenkins JLShen HGreen MRKielkopf CL Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65.; The Journal of biological chemistry; Vol 283(48). 2008 Nov 28. Manceau VKielkopf CLSobel AMaucuer A Different requirements of the kinase and UHM domains of KIS for its nuclear localization and binding to splicing factors.; Journal of molecular biology; Vol 381(3). 2008 Sep 5. Lin YKielkopf CL X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines.; Biochemistry; Vol 47(20). 2008 May 20. Kumar AOSwenson MCBenning MMKielkopf CL Structure of the central RNA recognition motif of human TIA-1 at 1.95A resolution.; Biochemical and biophysical research communications; Vol 367(4). 2008 Mar 21. Swenson MCParanawithana SRMiller PSKielkopf CL Structure of a DNA repair substrate containing an alkyl interstrand cross-link at 1.65 A resolution.; Biochemistry; Vol 46(15). 2007 Apr 17. Thickman KRSickmier EAKielkopf CL Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif.; Journal of molecular biology; Vol 366(3). 2007 Feb 23. Sickmier EAFrato KEShen HParanawithana SRGreen MRKielkopf CL Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65.; Molecular cell; Vol 23(1). 2006 Jul 7. Sickmier EAFrato KEKielkopf CL Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering.; Acta crystallographica. Section F, Structural biology and crystallization communications; Vol 62(Pt 5). 2006 May 1. Thickman KRSwenson MCKabogo JMGryczynski ZKielkopf CL Multiple U2AF65 binding sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA splicing factors.; Journal of molecular biology; Vol 356(3). 2006 Feb 24. Manceau VSwenson MLe Caer JPSobel AKielkopf CLMaucuer A Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65.; The FEBS journal; Vol 273(3). 2006 Feb. Sickmier EABrekasis DParanawithana SBonanno JBPaget MSBurley SKKielkopf CL X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing.; Structure (London, England : 1993); Vol 13(1). 2005 Jan. Kielkopf CLLücke SGreen MR U2AF homology motifs: protein recognition in the RRM world.; Genes & development; Vol 18(13). 2004 Jul 1. Kielkopf CLBurley SK X-ray structures of threonine aldolase complexes: structural basis of substrate recognition.; Biochemistry; Vol 41(39). 2002 Oct 1. Kielkopf CLRodionova NAGreen MRBurley SK A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer.; Cell; Vol 106(5). 2001 Sep 7. Kielkopf CLDing SKuhn PRees DC Conformational flexibility of B-DNA at 0.74 A resolution: d(CCAGTACTGG)(2).; Journal of molecular biology; Vol 296(3). 2000 Feb 25. Kielkopf CLErkkila KEHudson BPBarton JKRees DC Structure of a photoactive rhodium complex intercalated into DNA.; Nature structural biology; Vol 7(2). 2000 Feb. Kielkopf CLBremer REWhite SSzewczyk JWTurner JMBaird EEDervan PBRees DC Structural effects of DNA sequence on T.A recognition by hydroxypyrrole/pyrrole pairs in the minor groove.; Journal of molecular biology; Vol 295(3). 2000 Jan 21. Kielkopf CLWhite SSzewczyk JWTurner JMBaird EEDervan PBRees DC A structural basis for recognition of A.T and T.A base pairs in the minor groove of B-DNA.; Science (New York, N.Y.); Vol 282(5386). 1998 Oct 2. Kielkopf CLBaird EEDervan PBRees DC Structural basis for G.C recognition in the DNA minor groove.; Nature structural biology; Vol 5(2). 1998 Feb.