Recent Publications

• 1. Dumont ME
  2. Konopka JB
  Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast α-Factor Receptor.; Biomolecules; Vol 12(6). 2022 May 30.
• 1. Connelly SM
  2. Sridharan R
  3. Naider F
  4. Dumont ME
  Oligomerization of Yeast α-factor Receptor Detected by Fluorescent Energy Transfer Between Ligands.; Biophysical journal. 2021 Oct 07.
• 1. Zhu H
  2. Mathew E
  3. Connelly SM
  4. Zuber J
  5. Sullivan M
  6. Piepenbrink MS
  7. Kobie JJ
  8. Dumont ME
  Identification of variant HIV envelope proteins with enhanced affinities for precursors to anti-gp41 broadly neutralizing antibodies.; PloS one; Vol 14(9). 2019.
• 1. Mathew E
  2. Zhu H
  3. Connelly SM
  4. Sullivan MA
  5. Brewer MG
  6. Piepenbrink MS
  7. Kobie JJ
  8. Dewhurst S
  9. Dumont ME
  Display of the HIV envelope protein at the yeast cell surface for immunogen development.; PloS one; Vol 13(10). 2018.
• 1. Clark KM
  2. Jenkins JL
  3. Fedoriw N
  4. Dumont ME
  Human CaaX protease ZMPSTE24 expressed in yeast: Structure and inhibition by HIV protease inhibitors.; Protein science : a publication of the Protein Society; Vol 26(2). 2017 Feb.
• 1. Coudray N
  2. L Seyler S
  3. Lasala R
  4. Zhang Z
  5. Clark KM
  6. Dumont ME
  7. Rohou A
  8. Beckstein O
  9. Stokes DL
  Structure of the SLC4 transporter Bor1p in an inward-facing conformation.; Protein science : a publication of the Protein Society; Vol 26(1). 2017 Jan.
• 1. Sridharan R
  2. Connelly SM
  3. Naider F
  4. Dumont ME
  Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein Coupled Receptor in Yeast.; The Journal of biological chemistry. 2016 Sep 19.
• 1. Coudray N
  2. Lasala R
  3. Zhang Z
  4. Clark KM
  5. Dumont ME
  6. Stokes DL
  Deducing the Symmetry of Helical Assemblies: Applications to Membrane Proteins.; Journal of structural biology. 2016 May 30.
• 1. Zhu G
  2. Koszelak-Rosenblum M
  3. Connelly SM
  4. Dumont ME
  5. Malkowski MG
  The Crystal Structure of an Integral Membrane Fatty Acid α-Hydroxylase.; The Journal of biological chemistry; Vol 290(50). 2015 Dec 11.
• 1. Zuber J
  2. Danial SA
  3. Connelly SM
  4. Naider F
  5. Dumont ME
  Identification of destabilizing and stabilizing mutations of Ste2p, a G protein-coupled receptor in Saccharomyces cerevisiae.; Biochemistry; Vol 54(9). 2015 Mar 10.
• 1. Mathew E
  2. Dumont ME
  A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs.; Methods in enzymology; Vol 557. 2015.
• 1. Cohen LS
  2. Arshava B
  3. Kauffman S
  4. Mathew E
  5. Fracchiolla KE
  6. Ding FX
  7. Dumont ME
  8. Becker JM
  9. Naider F
  Guided reconstitution of membrane protein fragments.; Biopolymers; Vol 102(1). 2014 Jan.
• 1. Sridharan R
  2. Zuber J
  3. Connelly SM
  4. Mathew E
  5. Dumont ME
  Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors.; Biochimica et biophysica acta; Vol 1838(1 Pt A). 2014 Jan.
• 1. Pryor EE
  2. Horanyi PS
  3. Clark KM
  4. Fedoriw N
  5. Connelly SM
  6. Koszelak-Rosenblum M
  7. Zhu G
  8. Malkowski MG
  9. Wiener MC
  10. Dumont ME
  Structure of the integral membrane protein CAAX protease Ste24p.; Science (New York, N.Y.); Vol 339(6127). 2013 Mar 29.
• 1. Pieper U
  2. Schlessinger A
  3. Kloppmann E
  4. Chang GA
  5. Chou JJ
  6. Dumont ME
  7. Fox BG
  8. Fromme P
  9. Hendrickson WA
  10. Malkowski MG
  11. Rees DC
  12. Stokes DL
  13. Stowell MH
  14. Wiener MC
  15. Rost B
  16. Stroud RM
  17. Stevens RC
  18. Sali A
  Coordinating the impact of structural genomics on the human α-helical transmembrane proteome.; Nature structural & molecular biology; Vol 20(2). 2013 Feb.
• 1. Mathew E
  2. Ding FX
  3. Naider F
  4. Dumont ME
  Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast.; Protein engineering, design & selection : PEDS; Vol 26(1). 2013 Jan.
• 1. Gehret AU
  2. Connelly SM
  3. Dumont ME
  Functional and physical interactions among Saccharomyces cerevisiae α-factor receptors.; Eukaryotic cell; Vol 11(10). 2012 Oct.
• 1. Lesoine JF
  2. Venkataraman PA
  3. Maloney PC
  4. Dumont ME
  5. Novotny L
  Nanochannel-based single molecule recycling.; Nano letters; Vol 12(6). 2012 Jun 13.
• 1. Taslimi A
  2. Mathew E
  3. Celić A
  4. Wessel S
  5. Dumont ME
  Identifying functionally important conformational changes in proteins: activation of the yeast α-factor receptor Ste2p.; Journal of molecular biology; Vol 418(5). 2012 May 18.
• 1. Kang DC
  2. Venkataraman PA
  3. Dumont ME
  4. Maloney PC
  Oligomeric state of the oxalate transporter, OxlT.; Biochemistry; Vol 50(39). 2011 Oct 4.
• 1. Mathew E
  2. Bajaj A
  3. Connelly SM
  4. Sargsyan H
  5. Ding FX
  6. Hajduczok AG
  7. Naider F
  8. Dumont ME
  Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p.; Journal of molecular biology; Vol 409(4). 2011 Jun 17.
• 1. Tantry S
  2. Ding FX
  3. Dumont M
  4. Becker JM
  5. Naider F
  Binding of fluorinated phenylalanine alpha-factor analogues to Ste2p: evidence for a cation-pi binding interaction between a peptide ligand and its cognate G protein-coupled receptor.; Biochemistry; Vol 49(24). 2010 Jun 22.
• 1. Clark KM
  2. Fedoriw N
  3. Robinson K
  4. Connelly SM
  5. Randles J
  6. Malkowski MG
  7. DeTitta GT
  8. Dumont ME
  Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.; Protein expression and purification; Vol 71(2). 2010 Jun.
• 1. Shi S
  2. Notenboom S
  3. Dumont ME
  4. Ballatori N
  Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast.; Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology; Vol 25(2-3). 2010.
• 1. Chloupková M
  2. Pickert A
  3. Lee JY
  4. Souza S
  5. Trinh YT
  6. Connelly SM
  7. Dumont ME
  8. Dean M
  9. Urbatsch IL
  Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity.; Biochemistry; Vol 46(27). 2007 Jul 10.
• 1. Bajaj A
  2. Connelly SM
  3. Gehret AU
  4. Naider F
  5. Dumont ME
  Role of extracellular charged amino acids in the yeast alpha-factor receptor.; Biochimica et biophysica acta; Vol 1773(6). 2007 Jun.
• 1. White MA
  2. Clark KM
  3. Grayhack EJ
  4. Dumont ME
  Characteristics affecting expression and solubilization of yeast membrane proteins.; Journal of molecular biology; Vol 365(3). 2007 Jan 19.
• 1. Wang Z
  2. Stalcup LD
  3. Harvey BJ
  4. Weber J
  5. Chloupkova M
  6. Dumont ME
  7. Dean M
  8. Urbatsch IL
  Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8.; Biochemistry; Vol 45(32). 2006 Aug 15.
• 1. Gehret AU
  2. Bajaj A
  3. Naider F
  4. Dumont ME
  Oligomerization of the yeast alpha-factor receptor: implications for dominant negative effects of mutant receptors.; The Journal of biological chemistry; Vol 281(30). 2006 Jul 28.
• 1. Gelperin DM
  2. White MA
  3. Wilkinson ML
  4. Kon Y
  5. Kung LA
  6. Wise KJ
  7. Lopez-Hoyo N
  8. Jiang L
  9. Piccirillo S
  10. Yu H
  11. Gerstein M
  12. Dumont ME
  13. Phizicky EM
  14. Snyder M
  15. Grayhack EJ
  Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.; Genes & development; Vol 19(23). 2005 Dec 1.
• 1. Bajaj A
  2. Celić A
  3. Ding FX
  4. Naider F
  5. Becker JM
  6. Dumont ME
  A fluorescent alpha-factor analogue exhibits multiple steps on binding to its G protein coupled receptor in yeast.; Biochemistry; Vol 43(42). 2004 Oct 26.
• 1. Alexandrov A
  2. Vignali M
  3. LaCount DJ
  4. Quartley E
  5. de Vries C
  6. De Rosa D
  7. Babulski J
  8. Mitchell SF
  9. Schoenfeld LW
  10. Fields S
  11. Hol WG
  12. Dumont ME
  13. Phizicky EM
  14. Grayhack EJ
  A facile method for high-throughput co-expression of protein pairs.; Molecular & cellular proteomics : MCP; Vol 3(9). 2004 Sep.
• 1. Roginskaya M
  2. Connelly SM
  3. Kim KS
  4. Patel D
  5. Dumont ME
  Effects of mutations in the N terminal region of the yeast G protein alpha-subunit Gpa1p on signaling by pheromone receptors.; Molecular genetics and genomics : MGG; Vol 271(2). 2004 Mar.
• 1. Celić A
  2. Connelly SM
  3. Martin NP
  4. Dumont ME
  Intensive mutational analysis of G protein-coupled receptors in yeast.; Methods in molecular biology (Clifton, N.J.); Vol 237. 2004.
• 1. Celić A
  2. Martin NP
  3. Son CD
  4. Becker JM
  5. Naider F
  6. Dumont ME
  Sequences in the intracellular loops of the yeast pheromone receptor Ste2p required for G protein activation.; Biochemistry; Vol 42(10). 2003 Mar 18.
• 1. Connelly SM
  2. Yuan J
  3. Ding FX
  4. Naider F
  5. Becker JM
  6. Dumont ME
  Effects of Inserting Intracellular Loops of the Yeast a-factor Receptor, Ste3p, into the a-factor Receptor, Ste2p; Yeast. 2003 Jan 01.
• 1. Arevalo E
  2. Estephan R
  3. Madeo J
  4. Arshava B
  5. Dumont ME
  6. Becker JM
  7. Naider F
  Biosynthesis and Biophysical Analysis of Domains of a Yeast G protein-coupled receptor; Biopolymers (Peptide Science). 2003 Jan 01.
• 1. Martin NP
  2. Celić A
  3. Dumont ME
  Mutagenic mapping of helical structures in the transmembrane segments of the yeast alpha-factor receptor.; Journal of molecular biology; Vol 317(5). 2002 Apr 12.
• 1. Erlenbach I
  2. Kostenis E
  3. Schmidt C
  4. Serradeil-Le Gal C
  5. Raufaste D
  6. Dumont ME
  7. Pausch MH
  8. Wess J
  Single amino acid substitutions and deletions that alter the G protein coupling properties of the V2 vasopressin receptor identified in yeast by receptor random mutagenesis.; The Journal of biological chemistry; Vol 276(31). 2001 Aug 3.
• 1. Sommers CM
  2. Martin NP
  3. Akal-Strader A
  4. Becker JM
  5. Naider F
  6. Dumont ME
  A limited spectrum of mutations causes constitutive activation of the yeast alpha-factor receptor.; Biochemistry; Vol 39(23). 2000 Jun 13.
• 1. Leavitt LM
  2. Macaluso CR
  3. Kim KS
  4. Martin NP
  5. Dumont ME
  Dominant negative mutations in the alpha-factor receptor, a G protein-coupled receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae.; Molecular & general genetics : MGG; Vol 261(6). 1999 Jul.
• 1. Martin NP
  2. Leavitt LM
  3. Sommers CM
  4. Dumont ME
  Assembly of G protein-coupled receptors from fragments: identification of functional receptors with discontinuities in each of the loops connecting transmembrane segments.; Biochemistry; Vol 38(2). 1999 Jan 12.
• 1. Sommers CM
  2. Dumont ME
  Genetic Approaches for Studying the Structure and Function of G Protein-Coupled receptors in Yeast in Structure-Function Analysis of G Protein-Coupled Receptors; ( J. Wess, ed.) Vol. III of Receptor Biochemistry and Methodology, , John Wiley and Sons, New York. 1999 Jan 01.
• 1. Rebbeor JF
  2. Connolly GC
  3. Dumont ME
  4. Ballatori N
  ATP-dependent transport of reduced glutathione on YCF1, the yeast orthologue of mammalian multidrug resistance associated proteins.; The Journal of biological chemistry; Vol 273(50). 1998 Dec 11.
• 1. Rebbeor JF
  2. Connolly GC
  3. Dumont ME
  4. Ballatori N
  ATP-dependent transport of reduced glutathione in yeast secretory vesicles.; The Biochemical journal; Vol 334 ( Pt 3). 1998 Sep 15.
• 1. Pollock WB
  2. Rosell FI
  3. Twitchett MB
  4. Dumont ME
  5. Mauk AG
  Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition.; Biochemistry; Vol 37(17). 1998 Apr 28.
• 1. Sommers CM
  2. Dumont ME
  Genetic interactions among the transmembrane segments of the G protein coupled receptor encoded by the yeast STE2 gene.; Journal of molecular biology; Vol 266(3). 1997 Feb 28.
• 1. Wang X
  2. Dumont ME
  3. Sherman F
  Sequence requirements for mitochondrial import of yeast cytochrome c.; The Journal of biological chemistry; Vol 271(12). 1996 Mar 22.
• 1. Dumont ME
  2. Corin AF
  3. Campbell GA
  Noncovalent binding of heme induces a compact apocytochrome c structure.; Biochemistry; Vol 33(23). 1994 Jun 14.
• 1. Dumont ME
  2. Schlichter JB
  3. Cardillo TS
  4. Hayes MK
  5. Bethlendy G
  6. Sherman F
  CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c.; Molecular and cellular biology; Vol 13(10). 1993 Oct.