Golisano Children's Hospital / Pediatric Nephrology / Instructions for Urine Testing Instructions for Urine Testing Fill the test tube 1/2 to 1/3 with urine. Add without shaking 10 drops of sulfosalicylic acid (SSA). Read the reaction as follows: Negative – No change in appearance of urine Trace – Very faint cloudiness 1+ – Moderate cloudiness through which newspaper print can be read 2+ – More marked cloudiness through which heavy black lines on a white paper are still visible 3+ – White cloud through which black lines are not visible 4+ – Fluffy white material which quickly sinks to the bottom of the tube Please note: SSA acid is 10% solution for this.